| Fred Hutch (FHCRC) | 501(c)(3) | Seattle, WA | $943 million |
| Henry M Jackson Foundation For The Advancement of Military Medicine | 501(c)(3) | Bethesda, MD | $569 million |
| City of Hope | 501(c)(3) | Duarte, CA | $479 million |
| The Michael J. Fox Foundation for PARKINSON'S Research | 501(c)(3) | New York, NY | $353 million |
| Patient Access Network Foundation | 501(c)(3) | Washington, DC | $310 million |
| Cold Spring Harbor Laboratory | 501(c)(3) | Cold Spring Harbor, NY | $202 million |
| National Multiple Sclerosis Society | 501(c)(3) | New York, NY | $146 million |
| American Technion Society | 501(c)(3) | New York, NY | $98 million |
| Helen Keller International (HKI) | 501(c)(3) | New York, NY | $89 million |
| Children's National Hospital Foundation | 501(c)(3) | Washington, DC | $82 million |
| Pan-Massachusetts Challenge | 501(c)(3) | Needham, MA | $66 million |
| THE Breast Cancer Research Foundation | 501(c)(3) | New York, NY | $64 million |
| Arthritis Foundation | 501(c)(3) | Atlanta, GA | $63 million |
| Parkinsons Disease Foundation Incorporated (PDF) | 501(c)(3) | Miami, FL | $50 million |
| National Kidney Foundation (NKF) | 501(c)(3) | New York, NY | $49 million |
| Lupus Research Alliance (ALR) | 501(c)(3) | New York, NY | $44 million |
| National Organization for Rare Disorders (NORD) | 501(c)(3) | Quincy, MA | $43 million |
| Foundation Fighting Blindness (FFB) | 501(c)(3) | Columbia, MD | $42 million |
| Cancer Research Institute (CRI) | 501(c)(3) | New York, NY | $35 million |
| Roswell Park Alliance Foundation | 501(c)(3) | Buffalo, NY | $33 million |
| The V Foundation | 501(c)(3) | Cary, NC | $32 million |
| Multiple Myeloma Research Foundation (MMRF) | 501(c)(3) | Norwalk, CT | $26 million |
| Gay Men's Health Crisis | 501(c)(3) | New York, NY | $23 million |
| Be The Match | 501(c)(3) | Minneapolis, MN | $21 million |
| Damon Runyon Cancer Research Foundation | 501(c)(3) | New York, NY | $20 million |
| St Baldrick's Foundation (SBF) | 501(c)(3) | Monrovia, CA | $19 million |
| Children's Tumor Foundation | 501(c)(3) | New York, NY | $16 million |
| Lupus Foundation of America | 501(c)(3) | Washington, DC | $15 million |
| American Parkinson Disease Association (APDA) | 501(c)(3) | Staten Island, NY | $14 million |
| Colorectal Cancer Alliance (CCA) | 501(c)(3) | Washington, DC | $14 million |
| American Institute for Cancer Research | 501(c)(3) | Arlington, VA | $14 million |
| GO2 Foundation For Lung Cancer (ALCF) | 501(c)(3) | Washington, DC | $13 million |
| National Foundation for Cancer Research (NFCR) | 501(c)(3) | Rockville, MD | $10 million |
| TSC Alliance | 501(c)(3) | Silver Spring, MD | $9.0 million |
| National Brain Tumor Society (NBTS) | 501(c)(3) | Newton, MA | $8.9 million |
| American Psychiatric Association Foundation (APAF) | 501(c)(3) | Washington, DC | $7.7 million |
| CURE Childhood Cancer | 501(c)(3) | Atlanta, GA | $7.5 million |
| American Brain Tumor Association | 501(c)(3) | Chicago, IL | $6.2 million |
| Pediatric Brain Tumor Foundation of the United States | 501(c)(3) | Atlanta, GA | $5.9 million |
| Pedal the Cause | 501(c)(3) | Saint Louis, MO | $5.9 million |
| Bone Health and Osteoporosis Foundation (OF) | 501(c)(3) | Arlington, VA | $4.4 million |
| Nephcure Kidney International (NKI) | 501(c)(3) | King of Prussia, PA | $3.9 million |
| National Kidney Foundation of Illinois (NKF) | 501(c)(3) | Chicago, IL | $2.1 million |
| Foundation for Biomedical Research (FBR) | 501(c)(3) | Washington, DC | $1.8 million |
| Cooley's Anemia Foundation | 501(c)(3) | New York, NY | $1.0 million |
| Vince Lombardi Cancer Foundation (VLCF) | 501(c)(3) | Milwaukee, WI | $122,389 |
| American Institutes for Research in the Behavioral Sciences | 501(c)(3) | Arlington, VA | $373 million |
| New Mexico State University Foundation | 501(c)(3) | Las Cruces, NM | $111 million |
| The Geneva Foundation | 501(c)(3) | Tacoma, WA | $79 million |
| BrightFocus Foundation | 501(c)(3) | Clarksburg, MD | $50 million |
| Community Services for Autistic Adults (CSAAC) | 501(c)(3) | Montgomery Village, MD | $36 million |
| W.W. Caruth Jr. Foundation | 501(c)(3) | Dallas, TX | $32 million |
| Conquer Cancer and Conquer Cancer FDTN | 501(c)(3) | Alexandria, VA | $24 million |
| Cure Pancreatic Cancer Lustgarten Foundation | 501(c)(3) | Woodbury, NY | $24 million |
| Orthopaedic Foundation | 501(c)(3) | Los Angeles, CA | $22 million |
| MetroHealth Foundation | 501(c)(3) | Cleveland, OH | $21 million |
| Epilepsy Foundation of America | 501(c)(3) | Bowie, MD | $19 million |
| Lymphoma Research Foundation (LRF) | 501(c)(3) | New York, NY | $16 million |
| Interstitial Cystitis Association of America (ICA) | 501(c)(3) | Mclean, VA | $745,097 |
| American Heart Association (AHA) | 501(c)(3) | Dallas, TX | $803 million |
| American Cancer Society (ACS) | 501(c)(3) | Kennesaw, GA | $734 million |
| Alzheimer's Association | 501(c)(3) | Chicago, IL | $502 million |
| The Leukemia & Lymphoma Society | 501(c)(3) | Overland Park, KS | $477 million |
| Population Services International (PSI) | 501(c)(3) | Washington, DC | $442 million |
| Cystic Fibrosis Foundation | 501(c)(3) | Bethesda, MD | $417 million |
| Juvenile Diabetes Research Foundation (JDRF) | 501(c)(3) | New York, NY | $223 million |
| Susan G Komen | 501(c)(3) | Dallas, TX | $87 million |
| Crohn's and Colitis Foundation (CCFA) | 501(c)(3) | New York, NY | $78 million |
| Riley Children's Endowment | 501(c)(3) | Indianapolis, IN | $68 million |
| Muscular Dystrophy Association | 501(c)(3) | Chicago, IL | $63 million |
| Pancreatic Cancer Action Network (PCAN) | 501(c)(3) | Manhattan Beach, CA | $52 million |
| Autism Speaks | 501(c)(3) | Princeton, NJ | $42 million |
| ALS Association (ALS) | 501(c)(3) | Arlington, VA | $39 million |
| National CHILDREN'S Cancer Society (NCCS) | 501(c)(3) | St Louis, MO | $30 million |
| Memorial Medical Center Foundation | 501(c)(3) | Long Beach, CA | $15 million |
| National Kidney Foundation Indiana | 501(c)(3) | Indianapolis, IN | $583,478 |
| Children's Medical Center Foundation | 501(c)(3) | Dallas, TX | $324 million |
| Pef Israel Endowment Funds | 501(c)(3) | New York, NY | $172 million |
| University of Texas Foundation (UTXF) | 501(c)(3) | Austin, TX | $47 million |
| Kessler Foundation | 501(c)(3) | East Hanover, NJ | $15 million |
| The Tommy Fuss Fund | 501(c)(3) | Wellesley, MA | $7.8 million |
| American Psychological Foundation (APF) | 501(c)(3) | Washington, DC | $7.3 million |
| Smith-Kettlewell Eye Research Institute (SKERI) | 501(c)(3) | San Francisco, CA | $5.6 million |
| Pediatric Epilepsy Research Foundation (PERF) | 501(c)(3) | Dallas, TX | $5.4 million |
| The Broad Institute | 501(c)(3) | Cambridge, MA | $1.3 billion |
| Health Research Incorporated (HRI) | 501(c)(3) | Menands, NY | $1.3 billion |
| Mtfbiologics (MTF) | 501(c)(3) | Edison, NJ | $462 million |
| Be the Match | 501(c)(3) | Minneapolis, MN | $445 million |
| Beckman Research Institute of the City of Hope (BRI) | 501(c)(3) | Duarte, CA | $339 million |
| Good Days | 501(c)(3) | Frisco, TX | $323 million |
| Cook Children's Health Foundation | 501(c)(3) | Fort Worth, TX | $259 million |
| ASU Foundation | 501(c)(3) | Tempe, AZ | $233 million |
| Research Foundation for Mental Hygiene (RFMH) | 501(c)(3) | Menands, NY | $207 million |
| Robert W Woodruff Health Sciences Center Fund | 501(c)(3) | Atlanta, GA | $199 million |
| Public Health Institute (PHI) | 501(c)(3) | Oakland, CA | $194 million |
| Moffitt Cancer Center (MCC) | 501(c)(3) | Tampa, FL | $175 million |
| The Translational Genomics Research Institute | 501(c)(3) | Phoenix, AZ | $175 million |
| Parker Institute for Cancer Immunotherapy | 501(c)(3) | San Francisco, CA | $170 million |
| Salk Institute for Biological Studies | 501(c)(3) | La Jolla, CA | $168 million |
| University of Connecticut Foundation | 501(c)(3) | Storrs, CT | $151 million |
| Emory Children's Pediatric Institute | 501(c)(3) | Atlanta, GA | $139 million |
| Southwestern Medical Foundation | 501(c)(3) | Dallas, TX | $133 million |
| Stony Brook Foundation | 501(c)(3) | Stony Brook, NY | $129 million |
| WMC New York | 501(c)(3) | Valhalla, NY | $127 million |
| Sanford Burnham Prebys Medical Discovery Institute | 501(c)(3) | La Jolla, CA | $124 million |
| Fidelity Non-Profit Management Foundation | 501(c)(3) | Boston, MA | $111 million |
| OHSU Foundation | 501(c)(3) | Portland, OR | $108 million |
| Foundation for Food and Agriculture Research (FFAR) | 501(c)(3) | Washington, DC | $105 million |
| La Jolla Institute for Immunology (LIAI) | 501(c)(3) | La Jolla, CA | $102 million |
| Oklahoma Medical Research Foundation (OMRF) | 501(c)(3) | Oklahoma City, OK | $95 million |
| Ludwig Institute for Cancer Research | 501(c)(3) | New York, NY | $89 million |
| Licr Fund | 501(c)(3) | New York, NY | $86 million |
| Joslin Diabetes Center | 501(c)(3) | Boston, MA | $81 million |
| MCV Foundation | 501(c)(3) | Richmond, VA | $75 million |
| CancerCare Co-Payment Assistance Foundation (CCAF) | 501(c)(3) | New York, NY | $74 million |
| International Livestock Research Institute (ILRI) | 501(c)(3) | Alexandria, VA | $74 million |
| New York Genome Center (NYGC) | 501(c)(3) | New York, NY | $72 million |
| The Gog Foundation | 501(c)(3) | Edgewater, MD | $71 million |
| Benaroya Research Institute at Virginia Mason | 501(c)(3) | Seattle, WA | $67 million |
| Wistar Institute of Anatomy and Biology | 501(c)(3) | Philadelphia, PA | $67 million |
| Children's Hospital Colorado Health System | 501(c)(3) | Aurora, CO | $64 million |
| HJF Medical Research International (HJFMRI) | 501(c)(3) | Bethesda, MD | $62 million |
| Jupiter Medical Center Foundation | 501(c)(3) | Jupiter, FL | $61 million |
| California Pacific Medical Center Foundation (CPMCF) | 501(c)(3) | Sacramento, CA | $61 million |
| Children's Hospital Colorado Foundation | 501(c)(3) | Aurora, CO | $60 million |
| Texas Heart Institute | 501(c)(3) | Houston, TX | $58 million |
| TB Alliance (TB Alliance) | 501(c)(3) | New York, NY | $58 million |
| Magee-Womens Research Institute and Foundation (MWRI) | 501(c)(3) | Pittsburgh, PA | $54 million |
| Alzheimers Drug Discovery Foundation (ADDF) | 501(c)(3) | New York, NY | $53 million |
| Prostate Cancer Foundation | 501(c)(3) | Santa Monica, CA | $53 million |
| Baptist Health South Florida Foundation | 501(c)(3) | Coral Gables, FL | $52 million |
| California State University Long Beach Research Foundation | 501(c)(3) | Long Beach, CA | $49 million |
| Progeria Research Foundation (PRF) | 501(c)(3) | Peabody, MA | $49 million |
| Upmc Children's Hospital Foundation (CHPF) | 501(c)(3) | Pittsburgh, PA | $48 million |
| Hennepin Healthcare Research Institute (MMRF) | 501(c)(3) | Minneapolis, MN | $47 million |
| Advocate Charitable Foundation | 501(c)(3) | Downers Grove, IL | $44 million |
| Altius Institute for Biomedical Sciences | 501(c)(3) | Seattle, WA | $44 million |
| Head Injury Association | 501(c)(3) | Hauppauge, NY | $43 million |
| American Committee for Shaare Zedek Medical Center in Jerusalem | 501(c)(3) | New York, NY | $42 million |
| CH Neurology Foundation | 501(c)(3) | Boston, MA | $41 million |
| CHMC Surgical Foundation | 501(c)(3) | Boston, MA | $41 million |
| Buck Institute for Research on Aging | 501(c)(3) | Novato, CA | $40 million |
| Alliance for Clinical Trials in Oncology Foundation | 501(c)(3) | Chicago, IL | $39 million |
| Cerebral Palsy Association of Nassau County | 501(c)(3) | Roosevelt, NY | $39 million |
| Choc Foundation | 501(c)(3) | Orange, CA | $39 million |
| Nsabp Foundation | 501(c)(3) | Pittsburgh, PA | $38 million |
| Cardiovascular Research Foundation | 501(c)(3) | New York, NY | $37 million |
| The Mark Foundation for Cancer Research | 501(c)(3) | New York, NY | $37 million |
| Swedish Medical Center Foundation | 501(c)(3) | Seattle, WA | $36 million |
| The Lieber Institute (LIBD) | 501(c)(3) | Baltimore, MD | $35 million |